Construct: shRNA TRCN0000090734

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009606.1-849s1c1
DNA Barcode:
CCAGCCTTCCTTTATCGGTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Acta1 (11459)
Gene Description:
actin, alpha 1, skeletal muscle
Transcript:
RefSeq NM_009606.1 (NON-CURRENT)
Match location:
Position 849 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000090734)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGCCTTCCTTTATCGGTAT
Hairpin Sequence:
5'-CCGG-CCAGCCTTCCTTTATCGGTAT-CTCGAG-ATACCGATAAAGGAAGGCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGCCTTCCTTTATCGGTATCTCGAGATACCGATAAAGGAAGGCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGCCTTCCTTTATCGGTATCTCGAGATACCGATAAAGGAAGGCTGG-3'

Other clones with same target sequence:

(none)