Construct: shRNA TRCN0000090751

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027721.1-1196s1c1
DNA Barcode:
CTTCGGATGAAGACGGAGTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Katnal2 (71206)
Gene Description:
katanin p60 subunit A-like 2
Transcript:
RefSeq NM_027721.1 (NON-CURRENT)
Match location:
Position 1196 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000090751)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCGGATGAAGACGGAGTTA
Hairpin Sequence:
5'-CCGG-CTTCGGATGAAGACGGAGTTA-CTCGAG-TAACTCCGTCTTCATCCGAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCGGATGAAGACGGAGTTACTCGAGTAACTCCGTCTTCATCCGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCGGATGAAGACGGAGTTACTCGAGTAACTCCGTCTTCATCCGAAG-3'

Other clones with same target sequence:

(none)