Construct: shRNA TRCN0000090763

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021608.2-1196s1c1
DNA Barcode:
GCTGCTAAGATGGGACCAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dctn5 (59288)
Gene Description:
dynactin 5
Transcript:
RefSeq NM_021608.2 (NON-CURRENT)
Match location:
Position 1196 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000090763)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCTAAGATGGGACCAATA
Hairpin Sequence:
5'-CCGG-GCTGCTAAGATGGGACCAATA-CTCGAG-TATTGGTCCCATCTTAGCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCTAAGATGGGACCAATACTCGAGTATTGGTCCCATCTTAGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCTAAGATGGGACCAATACTCGAGTATTGGTCCCATCTTAGCAGC-3'

Other clones with same target sequence:

  1. TRCN0000327105