Construct: shRNA TRCN0000090806

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009446.1-661s1c1
DNA Barcode:
GCTTTCATGGTGGATAACGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tuba3a (22144)
Gene Description:
tubulin, alpha 3A
Transcript:
RefSeq NM_009446.1 (NON-CURRENT)
Match location:
Position 661 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000090806)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTTCATGGTGGATAACGAA
Hairpin Sequence:
5'-CCGG-GCTTTCATGGTGGATAACGAA-CTCGAG-TTCGTTATCCACCATGAAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTTCATGGTGGATAACGAACTCGAGTTCGTTATCCACCATGAAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTTCATGGTGGATAACGAACTCGAGTTCGTTATCCACCATGAAAGC-3'

Other clones with same target sequence:

(none)