Construct: shRNA TRCN0000090912

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008446.1-2352s1c1
DNA Barcode:
GCTAAGAAGATGACTCAGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kif4 (16571)
Gene Description:
kinesin family member 4
Transcript:
RefSeq NM_008446.1 (NON-CURRENT)
Match location:
Position 2352 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000090912)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTAAGAAGATGACTCAGAAT
Hairpin Sequence:
5'-CCGG-GCTAAGAAGATGACTCAGAAT-CTCGAG-ATTCTGAGTCATCTTCTTAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTAAGAAGATGACTCAGAATCTCGAGATTCTGAGTCATCTTCTTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTAAGAAGATGACTCAGAATCTCGAGATTCTGAGTCATCTTCTTAGC-3'

Other clones with same target sequence:

  1. TRCN0000309135