Construct: shRNA TRCN0000090971

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019391.1-1399s1c1
DNA Barcode:
CATGGGAAGTACGAGAAAGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lsp1 (16985)
Gene Description:
lymphocyte specific 1
Transcript:
RefSeq NM_019391.1 (NON-CURRENT)
Match location:
Position 1399 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000090971)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATGGGAAGTACGAGAAAGTA
Hairpin Sequence:
5'-CCGG-CATGGGAAGTACGAGAAAGTA-CTCGAG-TACTTTCTCGTACTTCCCATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATGGGAAGTACGAGAAAGTACTCGAGTACTTTCTCGTACTTCCCATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATGGGAAGTACGAGAAAGTACTCGAGTACTTTCTCGTACTTCCCATG-3'

Other clones with same target sequence:

  1. TRCN0000349435