Construct: shRNA TRCN0000090980

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_356184.3-3383s1c1
DNA Barcode:
CCAACGAATTTCACCTGTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Col6a4 (68553)
Gene Description:
collagen, type VI, alpha 4
Transcript:
RefSeq XM_356184.3 (NON-CURRENT)
Match location:
Position 3383 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000090980)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAACGAATTTCACCTGTTTA
Hairpin Sequence:
5'-CCGG-CCAACGAATTTCACCTGTTTA-CTCGAG-TAAACAGGTGAAATTCGTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAACGAATTTCACCTGTTTACTCGAGTAAACAGGTGAAATTCGTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAACGAATTTCACCTGTTTACTCGAGTAAACAGGTGAAATTCGTTGG-3'

Other clones with same target sequence:

(none)