Construct: shRNA TRCN0000090987

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011779.2-875s1c1
DNA Barcode:
GCTCTCCATGAAATGGACACT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Coro1c (23790)
Gene Description:
coronin, actin binding protein 1C
Transcript:
RefSeq NM_011779.2 (NON-CURRENT)
Match location:
Position 875 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000090987)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCTCCATGAAATGGACACT
Hairpin Sequence:
5'-CCGG-GCTCTCCATGAAATGGACACT-CTCGAG-AGTGTCCATTTCATGGAGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCTCCATGAAATGGACACTCTCGAGAGTGTCCATTTCATGGAGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCTCCATGAAATGGACACTCTCGAGAGTGTCCATTTCATGGAGAGC-3'

Other clones with same target sequence:

(none)