Construct: shRNA TRCN0000091135

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_203357.5-1164s1c1
DNA Barcode:
GCTGCTGTCTTATATTCCCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Myo15b (217328)
Gene Description:
myosin XVB
Transcript:
RefSeq XM_203357.5 (NON-CURRENT)
Match location:
Position 1164 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000091135)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCTGTCTTATATTCCCAA
Hairpin Sequence:
5'-CCGG-GCTGCTGTCTTATATTCCCAA-CTCGAG-TTGGGAATATAAGACAGCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCTGTCTTATATTCCCAACTCGAGTTGGGAATATAAGACAGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCTGTCTTATATTCCCAACTCGAGTTGGGAATATAAGACAGCAGC-3'

Other clones with same target sequence:

(none)