Construct: shRNA TRCN0000091140

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_138682.3-284s1c1
DNA Barcode:
CCACCATCAAGACCAAGCATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC212252 (212252)
Gene Description:
similar to Tuba6-prov protein
Transcript:
RefSeq XM_138682.3 (NON-CURRENT)
Match location:
Position 284 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000091140)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACCATCAAGACCAAGCATA
Hairpin Sequence:
5'-CCGG-CCACCATCAAGACCAAGCATA-CTCGAG-TATGCTTGGTCTTGATGGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACCATCAAGACCAAGCATACTCGAGTATGCTTGGTCTTGATGGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACCATCAAGACCAAGCATACTCGAGTATGCTTGGTCTTGATGGTGG-3'

Other clones with same target sequence:

(none)