Construct: shRNA TRCN0000091177

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_201245.1-1532s1c1
DNA Barcode:
GCCTGAGATACTACAGGGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mprip (26936)
Gene Description:
myosin phosphatase Rho interacting protein
Transcript:
RefSeq NM_201245.1 (NON-CURRENT)
Match location:
Position 1532 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000091177)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTGAGATACTACAGGGATT
Hairpin Sequence:
5'-CCGG-GCCTGAGATACTACAGGGATT-CTCGAG-AATCCCTGTAGTATCTCAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTGAGATACTACAGGGATTCTCGAGAATCCCTGTAGTATCTCAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTGAGATACTACAGGGATTCTCGAGAATCCCTGTAGTATCTCAGGC-3'

Other clones with same target sequence:

  1. TRCN0000318286