Construct: shRNA TRCN0000091200

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_485706.1-94s1c1
DNA Barcode:
CCAAATAAGAGGTTTCACTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC433972 (433972)
Gene Description:
similar to 3110007F17Rik protein
Transcript:
RefSeq XM_485706.1 (NON-CURRENT)
Match location:
Position 94 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000091200)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAATAAGAGGTTTCACTTT
Hairpin Sequence:
5'-CCGG-CCAAATAAGAGGTTTCACTTT-CTCGAG-AAAGTGAAACCTCTTATTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAATAAGAGGTTTCACTTTCTCGAGAAAGTGAAACCTCTTATTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAATAAGAGGTTTCACTTTCTCGAGAAAGTGAAACCTCTTATTTGG-3'

Other clones with same target sequence:

(none)