Construct: shRNA TRCN0000091283

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010617.1-5850s1c1
DNA Barcode:
GCAGATGAGAAGCATATCTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kif13a (16553)
Gene Description:
kinesin family member 13A
Transcript:
RefSeq NM_010617.1 (NON-CURRENT)
Match location:
Position 5850 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000091283)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGATGAGAAGCATATCTTA
Hairpin Sequence:
5'-CCGG-GCAGATGAGAAGCATATCTTA-CTCGAG-TAAGATATGCTTCTCATCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGATGAGAAGCATATCTTACTCGAGTAAGATATGCTTCTCATCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGATGAGAAGCATATCTTACTCGAGTAAGATATGCTTCTCATCTGC-3'

Other clones with same target sequence:

(none)