Construct: shRNA TRCN0000091295

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009932.2-1305s1c1
DNA Barcode:
CCGTCGGAGATGAAGATTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Col4a2 (12827)
Gene Description:
collagen, type IV, alpha 2
Transcript:
RefSeq NM_009932.2 (NON-CURRENT)
Match location:
Position 1305 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000091295)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTCGGAGATGAAGATTCAA
Hairpin Sequence:
5'-CCGG-CCGTCGGAGATGAAGATTCAA-CTCGAG-TTGAATCTTCATCTCCGACGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTCGGAGATGAAGATTCAACTCGAGTTGAATCTTCATCTCCGACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTCGGAGATGAAGATTCAACTCGAGTTGAATCTTCATCTCCGACGG-3'

Other clones with same target sequence:

(none)