Construct: shRNA TRCN0000091347

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008440.1-4746s1c1
DNA Barcode:
CCTGCCAGAACTTGACTCTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kif1a (16560)
Gene Description:
kinesin family member 1A
Transcript:
RefSeq NM_008440.1 (NON-CURRENT)
Match location:
Position 4746 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000091347)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGCCAGAACTTGACTCTAA
Hairpin Sequence:
5'-CCGG-CCTGCCAGAACTTGACTCTAA-CTCGAG-TTAGAGTCAAGTTCTGGCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGCCAGAACTTGACTCTAACTCGAGTTAGAGTCAAGTTCTGGCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGCCAGAACTTGACTCTAACTCGAGTTAGAGTCAAGTTCTGGCAGG-3'

Other clones with same target sequence:

(none)