Construct: shRNA TRCN0000091384

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013813.1-353s1c1
DNA Barcode:
GCTGCGAAACAGCTTGAATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Epb41l3 (13823)
Gene Description:
erythrocyte membrane protein band 4.1 like 3
Transcript:
RefSeq NM_013813.1 (NON-CURRENT)
Match location:
Position 353 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000091384)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCGAAACAGCTTGAATAT
Hairpin Sequence:
5'-CCGG-GCTGCGAAACAGCTTGAATAT-CTCGAG-ATATTCAAGCTGTTTCGCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCGAAACAGCTTGAATATCTCGAGATATTCAAGCTGTTTCGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCGAAACAGCTTGAATATCTCGAGATATTCAAGCTGTTTCGCAGC-3'

Other clones with same target sequence:

(none)