Construct: shRNA TRCN0000091393

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_486204.1-263s1c1
DNA Barcode:
GCAGTGTTTGTAGACCTGGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC434390 (434390)
Gene Description:
similar to alpha-tubulin
Transcript:
RefSeq XM_486204.1 (NON-CURRENT)
Match location:
Position 263 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000091393)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTGTTTGTAGACCTGGAA
Hairpin Sequence:
5'-CCGG-GCAGTGTTTGTAGACCTGGAA-CTCGAG-TTCCAGGTCTACAAACACTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTGTTTGTAGACCTGGAACTCGAGTTCCAGGTCTACAAACACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTGTTTGTAGACCTGGAACTCGAGTTCCAGGTCTACAAACACTGC-3'

Other clones with same target sequence:

(none)