Construct: shRNA TRCN0000091404

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_141326.4-7216s1c1
DNA Barcode:
GCCCACATCAAGGGAATCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Spnb5 (214545)
Gene Description:
spectrin beta 4
Transcript:
RefSeq XM_141326.4 (NON-CURRENT)
Match location:
Position 7216 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000091404)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCACATCAAGGGAATCAAA
Hairpin Sequence:
5'-CCGG-GCCCACATCAAGGGAATCAAA-CTCGAG-TTTGATTCCCTTGATGTGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCACATCAAGGGAATCAAACTCGAGTTTGATTCCCTTGATGTGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCACATCAAGGGAATCAAACTCGAGTTTGATTCCCTTGATGTGGGC-3'

Other clones with same target sequence:

(none)