Construct: shRNA TRCN0000091407

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_141326.4-738s1c1
DNA Barcode:
CCCTCTGTATAACCTCTCCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Spnb5 (214545)
Gene Description:
spectrin beta 4
Transcript:
RefSeq XM_141326.4 (NON-CURRENT)
Match location:
Position 738 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000091407)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTCTGTATAACCTCTCCTT
Hairpin Sequence:
5'-CCGG-CCCTCTGTATAACCTCTCCTT-CTCGAG-AAGGAGAGGTTATACAGAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTCTGTATAACCTCTCCTTCTCGAGAAGGAGAGGTTATACAGAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTCTGTATAACCTCTCCTTCTCGAGAAGGAGAGGTTATACAGAGGG-3'

Other clones with same target sequence:

(none)