Construct: shRNA TRCN0000091475

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144557.2-232s1c1
DNA Barcode:
AGAGACTTCAATCTTCGCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Myrip (245049)
Gene Description:
myosin VIIA and Rab interacting protein
Transcript:
RefSeq NM_144557.2 (NON-CURRENT)
Match location:
Position 232 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000091475)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAGACTTCAATCTTCGCAAA
Hairpin Sequence:
5'-CCGG-AGAGACTTCAATCTTCGCAAA-CTCGAG-TTTGCGAAGATTGAAGTCTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAGACTTCAATCTTCGCAAACTCGAGTTTGCGAAGATTGAAGTCTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAGACTTCAATCTTCGCAAACTCGAGTTTGCGAAGATTGAAGTCTCT-3'

Other clones with same target sequence:

(none)