Construct: shRNA TRCN0000091499

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016675.3-556s1c1
DNA Barcode:
GCGATATCTACAGTACCCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cldn2 (12738)
Gene Description:
claudin 2
Transcript:
RefSeq NM_016675.3 (NON-CURRENT)
Match location:
Position 556 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000091499)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGATATCTACAGTACCCTTT
Hairpin Sequence:
5'-CCGG-GCGATATCTACAGTACCCTTT-CTCGAG-AAAGGGTACTGTAGATATCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGATATCTACAGTACCCTTTCTCGAGAAAGGGTACTGTAGATATCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGATATCTACAGTACCCTTTCTCGAGAAAGGGTACTGTAGATATCGC-3'

Other clones with same target sequence:

(none)