Construct: shRNA TRCN0000091553

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025975.3-1416s1c1
DNA Barcode:
CCCAGAAAGTAATACATCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dynlt3 (67117)
Gene Description:
dynein light chain Tctex-type 3
Transcript:
RefSeq NM_025975.3 (NON-CURRENT)
Match location:
Position 1416 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000091553)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGAAAGTAATACATCAAA
Hairpin Sequence:
5'-CCGG-CCCAGAAAGTAATACATCAAA-CTCGAG-TTTGATGTATTACTTTCTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGAAAGTAATACATCAAACTCGAGTTTGATGTATTACTTTCTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGAAAGTAATACATCAAACTCGAGTTTGATGTATTACTTTCTGGG-3'

Other clones with same target sequence:

(none)