Construct: shRNA TRCN0000091567

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_193582.5-1246s1c1
DNA Barcode:
CACCATGAGAAAGCTGTGGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lin7a (108030)
Gene Description:
lin-7 homolog A (C. elegans)
Transcript:
RefSeq XM_193582.5 (NON-CURRENT)
Match location:
Position 1246 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000091567)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACCATGAGAAAGCTGTGGAA
Hairpin Sequence:
5'-CCGG-CACCATGAGAAAGCTGTGGAA-CTCGAG-TTCCACAGCTTTCTCATGGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACCATGAGAAAGCTGTGGAACTCGAGTTCCACAGCTTTCTCATGGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACCATGAGAAAGCTGTGGAACTCGAGTTCCACAGCTTTCTCATGGTG-3'

Other clones with same target sequence:

(none)