Construct: shRNA TRCN0000091577

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_145161.4-2698s1c1
DNA Barcode:
GCAGCTAATGACATGTTCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Col28a1 (213945)
Gene Description:
collagen, type XXVIII, alpha 1
Transcript:
RefSeq XM_145161.4 (NON-CURRENT)
Match location:
Position 2698 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000091577)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGCTAATGACATGTTCAAA
Hairpin Sequence:
5'-CCGG-GCAGCTAATGACATGTTCAAA-CTCGAG-TTTGAACATGTCATTAGCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGCTAATGACATGTTCAAACTCGAGTTTGAACATGTCATTAGCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGCTAATGACATGTTCAAACTCGAGTTTGAACATGTCATTAGCTGC-3'

Other clones with same target sequence:

(none)