Construct: shRNA TRCN0000091606

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_199473.1-1516s1c1
DNA Barcode:
CCTAGGACAGAAAGGTGACTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Col8a2 (329941)
Gene Description:
collagen, type VIII, alpha 2
Transcript:
RefSeq NM_199473.1 (NON-CURRENT)
Match location:
Position 1516 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000091606)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTAGGACAGAAAGGTGACTT
Hairpin Sequence:
5'-CCGG-CCTAGGACAGAAAGGTGACTT-CTCGAG-AAGTCACCTTTCTGTCCTAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTAGGACAGAAAGGTGACTTCTCGAGAAGTCACCTTTCTGTCCTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTAGGACAGAAAGGTGACTTCTCGAGAAGTCACCTTTCTGTCCTAGG-3'

Other clones with same target sequence:

(none)