Construct: shRNA TRCN0000091622

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009386.1-5003s1c1
DNA Barcode:
CGGCCATTTGAACGCAAATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tjp1 (21872)
Gene Description:
tight junction protein 1
Transcript:
RefSeq NM_009386.1 (NON-CURRENT)
Match location:
Position 5003 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000091622)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGCCATTTGAACGCAAATTT
Hairpin Sequence:
5'-CCGG-CGGCCATTTGAACGCAAATTT-CTCGAG-AAATTTGCGTTCAAATGGCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGCCATTTGAACGCAAATTTCTCGAGAAATTTGCGTTCAAATGGCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGCCATTTGAACGCAAATTTCTCGAGAAATTTGCGTTCAAATGGCCG-3'

Other clones with same target sequence:

  1. TRCN0000302326