Construct: shRNA TRCN0000091687

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_283319.4-1199s1c1
DNA Barcode:
GCGCGAGTATCAGGATCTCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Krt80 (74127)
Gene Description:
keratin 80
Transcript:
RefSeq XM_283319.4 (NON-CURRENT)
Match location:
Position 1199 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000091687)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGCGAGTATCAGGATCTCAT
Hairpin Sequence:
5'-CCGG-GCGCGAGTATCAGGATCTCAT-CTCGAG-ATGAGATCCTGATACTCGCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGCGAGTATCAGGATCTCATCTCGAGATGAGATCCTGATACTCGCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGCGAGTATCAGGATCTCATCTCGAGATGAGATCCTGATACTCGCGC-3'

Other clones with same target sequence:

  1. TRCN0000312166