Construct: shRNA TRCN0000091689

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013791.1-1020s1c1
DNA Barcode:
GCGGAGACAAATCTACACATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mkln1 (27418)
Gene Description:
muskelin 1, intracellular mediator containing kelch motifs
Transcript:
RefSeq NM_013791.1 (NON-CURRENT)
Match location:
Position 1020 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000091689)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGGAGACAAATCTACACATT
Hairpin Sequence:
5'-CCGG-GCGGAGACAAATCTACACATT-CTCGAG-AATGTGTAGATTTGTCTCCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGGAGACAAATCTACACATTCTCGAGAATGTGTAGATTTGTCTCCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGGAGACAAATCTACACATTCTCGAGAATGTGTAGATTTGTCTCCGC-3'

Other clones with same target sequence:

(none)