Construct: shRNA TRCN0000091696

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_204030.3-1981s1c1
DNA Barcode:
CAGAAGAGTATGAAGTGGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rnf19b (75234)
Gene Description:
ring finger protein 19B
Transcript:
RefSeq XM_204030.3 (NON-CURRENT)
Match location:
Position 1981 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000091696)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGAAGAGTATGAAGTGGAAT
Hairpin Sequence:
5'-CCGG-CAGAAGAGTATGAAGTGGAAT-CTCGAG-ATTCCACTTCATACTCTTCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGAAGAGTATGAAGTGGAATCTCGAGATTCCACTTCATACTCTTCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGAAGAGTATGAAGTGGAATCTCGAGATTCCACTTCATACTCTTCTG-3'

Other clones with same target sequence:

  1. TRCN0000334053