Construct: shRNA TRCN0000091697

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_204030.3-980s1c1
DNA Barcode:
GCACAAGAGGAATTTGGCTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rnf19b (75234)
Gene Description:
ring finger protein 19B
Transcript:
RefSeq XM_204030.3 (NON-CURRENT)
Match location:
Position 980 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000091697)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACAAGAGGAATTTGGCTAT
Hairpin Sequence:
5'-CCGG-GCACAAGAGGAATTTGGCTAT-CTCGAG-ATAGCCAAATTCCTCTTGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACAAGAGGAATTTGGCTATCTCGAGATAGCCAAATTCCTCTTGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACAAGAGGAATTTGGCTATCTCGAGATAGCCAAATTCCTCTTGTGC-3'

Other clones with same target sequence:

  1. TRCN0000334052