Construct: shRNA TRCN0000091803

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007735.1-5654s1c1
DNA Barcode:
CGGATGACAAAGACCAGATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Col4a4 (12829)
Gene Description:
collagen, type IV, alpha 4
Transcript:
RefSeq NM_007735.1 (NON-CURRENT)
Match location:
Position 5654 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000091803)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGATGACAAAGACCAGATTA
Hairpin Sequence:
5'-CCGG-CGGATGACAAAGACCAGATTA-CTCGAG-TAATCTGGTCTTTGTCATCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGATGACAAAGACCAGATTACTCGAGTAATCTGGTCTTTGTCATCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGATGACAAAGACCAGATTACTCGAGTAATCTGGTCTTTGTCATCCG-3'

Other clones with same target sequence:

(none)