Construct: shRNA TRCN0000091857

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_484897.2-303s1c1
DNA Barcode:
CCTTTCTCATATTGTGAACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Col6a3 (12835)
Gene Description:
collagen, type VI, alpha 3
Transcript:
RefSeq XM_484897.2 (NON-CURRENT)
Match location:
Position 303 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000091857)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTTCTCATATTGTGAACAT
Hairpin Sequence:
5'-CCGG-CCTTTCTCATATTGTGAACAT-CTCGAG-ATGTTCACAATATGAGAAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTTCTCATATTGTGAACATCTCGAGATGTTCACAATATGAGAAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTTCTCATATTGTGAACATCTCGAGATGTTCACAATATGAGAAAGG-3'

Other clones with same target sequence:

(none)