Construct: shRNA TRCN0000091890

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_484434.1-453s1c1
DNA Barcode:
CCAGGTGTATAGATGTGTCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC432918 (432918)
Gene Description:
similar to Filamin B (FLN-B) (Beta-filamin) (Actin-binding like protein) (ABP-280-like protein)
Transcript:
RefSeq XM_484434.1 (NON-CURRENT)
Match location:
Position 453 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000091890)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGGTGTATAGATGTGTCTA
Hairpin Sequence:
5'-CCGG-CCAGGTGTATAGATGTGTCTA-CTCGAG-TAGACACATCTATACACCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGGTGTATAGATGTGTCTACTCGAGTAGACACATCTATACACCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGGTGTATAGATGTGTCTACTCGAGTAGACACATCTATACACCTGG-3'

Other clones with same target sequence:

(none)