Construct: shRNA TRCN0000091958

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009447.2-1846s1c1
DNA Barcode:
GCGTCATTGAACACTGAGGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tuba4a (22145)
Gene Description:
tubulin, alpha 4A
Transcript:
RefSeq NM_009447.2 (NON-CURRENT)
Match location:
Position 1846 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000091958)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGTCATTGAACACTGAGGAT
Hairpin Sequence:
5'-CCGG-GCGTCATTGAACACTGAGGAT-CTCGAG-ATCCTCAGTGTTCAATGACGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGTCATTGAACACTGAGGATCTCGAGATCCTCAGTGTTCAATGACGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGTCATTGAACACTGAGGATCTCGAGATCCTCAGTGTTCAATGACGC-3'

Other clones with same target sequence:

(none)