Construct: shRNA TRCN0000092018

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_487408.1-1098s1c1
DNA Barcode:
CCACCTGATCGCCTGCACTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC385704 (385704)
Gene Description:
similar to coagulation factor V
Transcript:
RefSeq XM_487408.1 (NON-CURRENT)
Match location:
Position 1098 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000092018)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACCTGATCGCCTGCACTAA
Hairpin Sequence:
5'-CCGG-CCACCTGATCGCCTGCACTAA-CTCGAG-TTAGTGCAGGCGATCAGGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACCTGATCGCCTGCACTAACTCGAGTTAGTGCAGGCGATCAGGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACCTGATCGCCTGCACTAACTCGAGTTAGTGCAGGCGATCAGGTGG-3'

Other clones with same target sequence:

(none)