Construct: shRNA TRCN0000092077

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_140340.5-456s1c1
DNA Barcode:
GCTCCGAACAAATGTATGGGA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm4950 (240289)
Gene Description:
predicted pseudogene 4950; proteasome (prosome, macropain) subunit, beta type 3 pseudogene
Transcript:
RefSeq XM_140340.5 (NON-CURRENT)
Match location:
Position 456 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000092077)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCCGAACAAATGTATGGGA
Hairpin Sequence:
5'-CCGG-GCTCCGAACAAATGTATGGGA-CTCGAG-TCCCATACATTTGTTCGGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCCGAACAAATGTATGGGACTCGAGTCCCATACATTTGTTCGGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCCGAACAAATGTATGGGACTCGAGTCCCATACATTTGTTCGGAGC-3'

Other clones with same target sequence:

(none)