Construct: shRNA TRCN0000092098

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025830.3-3453s1c1
DNA Barcode:
GCCCTCTTGATTCAGTAGCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wwp2 (66894)
Gene Description:
WW domain containing E3 ubiquitin protein ligase 2
Transcript:
RefSeq NM_025830.3 (CURRENT)
Match location:
Position 3453 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000092098)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCTCTTGATTCAGTAGCTT
Hairpin Sequence:
5'-CCGG-GCCCTCTTGATTCAGTAGCTT-CTCGAG-AAGCTACTGAATCAAGAGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCTCTTGATTCAGTAGCTTCTCGAGAAGCTACTGAATCAAGAGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCTCTTGATTCAGTAGCTTCTCGAGAAGCTACTGAATCAAGAGGGC-3'

Other clones with same target sequence:

  1. TRCN0000302411