Construct: shRNA TRCN0000092179

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_483884.1-289s1c1
DNA Barcode:
GCAGGTGTTTAAGCAGGTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC432446 (432446)
Gene Description:
similar to hypothetical protein
Transcript:
RefSeq XM_483884.1 (NON-CURRENT)
Match location:
Position 289 (5UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000092179)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGGTGTTTAAGCAGGTAAA
Hairpin Sequence:
5'-CCGG-GCAGGTGTTTAAGCAGGTAAA-CTCGAG-TTTACCTGCTTAAACACCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGGTGTTTAAGCAGGTAAACTCGAGTTTACCTGCTTAAACACCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGGTGTTTAAGCAGGTAAACTCGAGTTTACCTGCTTAAACACCTGC-3'

Other clones with same target sequence:

(none)