Construct: shRNA TRCN0000092356

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_486404.1-426s1c1
DNA Barcode:
GATGAGAGATCCTTTCGGTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC434560 (434560)
Gene Description:
similar to bM64F17.3 (putative novel protein)
Transcript:
RefSeq XM_486404.1 (NON-CURRENT)
Match location:
Position 426 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000092356)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGAGAGATCCTTTCGGTAT
Hairpin Sequence:
5'-CCGG-GATGAGAGATCCTTTCGGTAT-CTCGAG-ATACCGAAAGGATCTCTCATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGAGAGATCCTTTCGGTATCTCGAGATACCGAAAGGATCTCTCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGAGAGATCCTTTCGGTATCTCGAGATACCGAAAGGATCTCTCATC-3'

Other clones with same target sequence:

(none)