Construct: shRNA TRCN0000092364

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_126674.5-896s1c1
DNA Barcode:
GCCCTGAGCTAGTGACTTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fbxl20 (72194)
Gene Description:
F-box and leucine-rich repeat protein 20
Transcript:
RefSeq XM_126674.5 (NON-CURRENT)
Match location:
Position 896 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000092364)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCTGAGCTAGTGACTTTAA
Hairpin Sequence:
5'-CCGG-GCCCTGAGCTAGTGACTTTAA-CTCGAG-TTAAAGTCACTAGCTCAGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCTGAGCTAGTGACTTTAACTCGAGTTAAAGTCACTAGCTCAGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCTGAGCTAGTGACTTTAACTCGAGTTAAAGTCACTAGCTCAGGGC-3'

Other clones with same target sequence:

  1. TRCN0000318022