Construct: shRNA TRCN0000092365

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_126674.5-1213s1c1
DNA Barcode:
CGGCTTCAAGTGTTGAGTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fbxl20 (72194)
Gene Description:
F-box and leucine-rich repeat protein 20
Transcript:
RefSeq XM_126674.5 (NON-CURRENT)
Match location:
Position 1213 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000092365)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGCTTCAAGTGTTGAGTCTT
Hairpin Sequence:
5'-CCGG-CGGCTTCAAGTGTTGAGTCTT-CTCGAG-AAGACTCAACACTTGAAGCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGCTTCAAGTGTTGAGTCTTCTCGAGAAGACTCAACACTTGAAGCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGCTTCAAGTGTTGAGTCTTCTCGAGAAGACTCAACACTTGAAGCCG-3'

Other clones with same target sequence:

(none)