Construct: shRNA TRCN0000092404

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033612.1-251s1c1
DNA Barcode:
CCCATGACTTATCGAGTGGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cela1 (109901)
Gene Description:
chymotrypsin-like elastase family, member 1
Transcript:
RefSeq NM_033612.1 (NON-CURRENT)
Match location:
Position 251 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000092404)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCATGACTTATCGAGTGGTT
Hairpin Sequence:
5'-CCGG-CCCATGACTTATCGAGTGGTT-CTCGAG-AACCACTCGATAAGTCATGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCATGACTTATCGAGTGGTTCTCGAGAACCACTCGATAAGTCATGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCATGACTTATCGAGTGGTTCTCGAGAACCACTCGATAAGTCATGGG-3'

Other clones with same target sequence:

  1. TRCN0000332347