Construct: shRNA TRCN0000092415

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_484543.1-257s1c1
DNA Barcode:
TCAAAGGGATTTCTGGAGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cstdc4 (433016)
Gene Description:
cystatin domain containing 4
Transcript:
RefSeq XM_484543.1 (NON-CURRENT)
Match location:
Position 257 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000092415)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCAAAGGGATTTCTGGAGAAA
Hairpin Sequence:
5'-CCGG-TCAAAGGGATTTCTGGAGAAA-CTCGAG-TTTCTCCAGAAATCCCTTTGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCAAAGGGATTTCTGGAGAAACTCGAGTTTCTCCAGAAATCCCTTTGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCAAAGGGATTTCTGGAGAAACTCGAGTTTCTCCAGAAATCCCTTTGA-3'

Other clones with same target sequence:

(none)