Construct: shRNA TRCN0000092443

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_194370.4-6052s1c1
DNA Barcode:
CCCAAATAAGTGGTCCTGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Adamtsl3 (269959)
Gene Description:
ADAMTS-like 3
Transcript:
RefSeq XM_194370.4 (NON-CURRENT)
Match location:
Position 6052 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000092443)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAAATAAGTGGTCCTGTTT
Hairpin Sequence:
5'-CCGG-CCCAAATAAGTGGTCCTGTTT-CTCGAG-AAACAGGACCACTTATTTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAAATAAGTGGTCCTGTTTCTCGAGAAACAGGACCACTTATTTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAAATAAGTGGTCCTGTTTCTCGAGAAACAGGACCACTTATTTGGG-3'

Other clones with same target sequence:

(none)