Construct: shRNA TRCN0000092470
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_201375.1-1858s1c1
- DNA Barcode:
- CCTGATGATTTGATCCCTGAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Kng2 (385643)
- Gene Description:
- kininogen 2
- Transcript:
- RefSeq NM_201375.1 (NON-CURRENT)
- Match location:
- Position 1858 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCTGATGATTTGATCCCTGAT
- Hairpin Sequence:
-
5'-CCGG-CCTGATGATTTGATCCCTGAT-CTCGAG-ATCAGGGATCAAATCATCAGG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCTGATGATTTGATCCCTGATCTCGAGATCAGGGATCAAATCATCAGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCTGATGATTTGATCCCTGATCTCGAGATCAGGGATCAAATCATCAGG-3'
Other clones with same target sequence:
(none)
