Construct: shRNA TRCN0000092477

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153195.1-319s1c1
DNA Barcode:
CATTCCTCACTCCAGGATAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fbxo7 (69754)
Gene Description:
F-box protein 7
Transcript:
RefSeq NM_153195.1 (NON-CURRENT)
Match location:
Position 319 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000092477)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATTCCTCACTCCAGGATAAT
Hairpin Sequence:
5'-CCGG-CATTCCTCACTCCAGGATAAT-CTCGAG-ATTATCCTGGAGTGAGGAATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATTCCTCACTCCAGGATAATCTCGAGATTATCCTGGAGTGAGGAATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATTCCTCACTCCAGGATAATCTCGAGATTATCCTGGAGTGAGGAATG-3'

Other clones with same target sequence:

(none)