Construct: shRNA TRCN0000092480

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_127466.6-467s1c1
DNA Barcode:
TGAAGCAACCTTTAAGAGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ube2ql1 (76980)
Gene Description:
ubiquitin-conjugating enzyme E2Q family-like 1
Transcript:
RefSeq XM_127466.6 (NON-CURRENT)
Match location:
Position 467 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000092480)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGAAGCAACCTTTAAGAGTTT
Hairpin Sequence:
5'-CCGG-TGAAGCAACCTTTAAGAGTTT-CTCGAG-AAACTCTTAAAGGTTGCTTCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGAAGCAACCTTTAAGAGTTTCTCGAGAAACTCTTAAAGGTTGCTTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGAAGCAACCTTTAAGAGTTTCTCGAGAAACTCTTAAAGGTTGCTTCA-3'

Other clones with same target sequence:

(none)