Construct: shRNA TRCN0000092518

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172485.2-5665s1c1
DNA Barcode:
GCAGAGTGTTAGCAAGGAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Thsd7b (210417)
Gene Description:
thrombospondin, type I, domain containing 7B
Transcript:
RefSeq NM_172485.2 (NON-CURRENT)
Match location:
Position 5665 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000092518)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAGTGTTAGCAAGGAAAT
Hairpin Sequence:
5'-CCGG-GCAGAGTGTTAGCAAGGAAAT-CTCGAG-ATTTCCTTGCTAACACTCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAGTGTTAGCAAGGAAATCTCGAGATTTCCTTGCTAACACTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAGTGTTAGCAAGGAAATCTCGAGATTTCCTTGCTAACACTCTGC-3'

Other clones with same target sequence:

(none)