Construct: shRNA TRCN0000092533

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_128716.4-666s1c1
DNA Barcode:
GCATAGCATAAGCGCACTGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fbxl17 (50758)
Gene Description:
F-box and leucine-rich repeat protein 17
Transcript:
RefSeq XM_128716.4 (NON-CURRENT)
Match location:
Position 666 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000092533)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATAGCATAAGCGCACTGTT
Hairpin Sequence:
5'-CCGG-GCATAGCATAAGCGCACTGTT-CTCGAG-AACAGTGCGCTTATGCTATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATAGCATAAGCGCACTGTTCTCGAGAACAGTGCGCTTATGCTATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATAGCATAAGCGCACTGTTCTCGAGAACAGTGCGCTTATGCTATGC-3'

Other clones with same target sequence:

(none)