Construct: shRNA TRCN0000092554

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_136108.4-11841s1c1
DNA Barcode:
CCGCACTGTGTTAAACCAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Huwe1 (59026)
Gene Description:
HECT, UBA and WWE domain containing 1
Transcript:
RefSeq XM_136108.4 (NON-CURRENT)
Match location:
Position 11841 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000092554)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGCACTGTGTTAAACCAGAT
Hairpin Sequence:
5'-CCGG-CCGCACTGTGTTAAACCAGAT-CTCGAG-ATCTGGTTTAACACAGTGCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGCACTGTGTTAAACCAGATCTCGAGATCTGGTTTAACACAGTGCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGCACTGTGTTAAACCAGATCTCGAGATCTGGTTTAACACAGTGCGG-3'

Other clones with same target sequence:

  1. TRCN0000327552